1x tn buffer (Thermo Fisher)

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Thermo Fisher 1x tn buffer
1x Tn Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tn+buffer/Tween+20/bio_rxiv__64898__2026__04__13__716336-296-69-54
Average 99 stars, based on 1 article reviews

1x tn buffer - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Live-cell imaging of circular and long non-coding RNAs associated to FUS pathological aggregates by Pepper fluorescent RNA
Article Snippet: .. SSC buffer was then replaced with TN buffer (Tris HCl pH 7.5 10 mM; NaCl 5 mM in nuclease free water), incubated at RT for 10 min. Lastly, biotinylated oligoes were stained with 1:200 diluted Alexa Fluor TM 568-conjugated streptavidin (InvitrogenTM S11226) incubated in 4% w/v BSA (Sigma-Aldrich, A2153)/TN buffer for 1-2 hours at room temperature in a humid box. .. When FISH was combined with IF, cells were washed twice with TN buffer and once with DPBS and then were incubated with primary antibodies (anti-FUS, Bioss Antibodies bs-2980R, anti-FUS, Santa Cruz sc-47711, anti-G3BP1, Sigma GW22382A, anti-G3BP1, Sigma PLA0231, anti-TIAR, BD Transduction MS BD 610352, anti-DCP1A, ab183709) diluted in 1% w/v BSA/DPBS for 1 hour at room temperature.

Article Title: Live-cell imaging of circular and long noncoding RNAs associated with FUS pathological aggregates by Pepper fluorescent RNA.
Article Snippet: .. SSC buffer was then replaced with TN buffer (Tris HCl pH 7.5 10 mM; NaCl 5 mM in nuclease-free water), incubated at RT for 10 min. Lastly, biotinylated oligos were stained with 1:200 diluted Alexa Fluor 568-conjugated streptavidin (Invitrogen S11226) incubated in 4% w/v BSA (SigmaAldrich, A2153)/TN buffer for 1–2 h at room temperature in a humid box. .. When FISH was combined with IF, cells were washed twice with TN buffer and once with DPBS and then incubated with primary antibodies (anti-FUS, Bioss Antibodies bs-2980R, anti-FUS, Santa Cruz sc-47711, anti-G3BP1, Sigma GW22382A, antiG3BP1, Sigma PLA0231, anti-TIAR, BD Transduction MS BD 610352, anti-DCP1A, ab183709) diluted in 1% w/v BSA/DPBS for 1 h at room temperature.

Article Title: AMBRA1 Controls Regulatory T-Cell Differentiation and Homeostasis Upstream of the FOXO3-FOXP3 Axis.
Article Snippet: .. For FOXP3 immunofluorescence, spinal cord slices were washed, permeabilized in 0.4% Triton/PBS for 30 minutes, blocked in 0.5% BSA/0,5% Casein in TN Buffer (hereafter ‘‘blocking buffer’’) for 1h and incubated overnight at 4 C with a mouse anti-FOXP3 antibody (eBioscience) 1:100 in blocking buffer. ..

Article Title: Lack of spatial segregation in the representation of pheromones and kairomones in the mouse medial amygdala
Article Snippet: .. Slides were then permeabilized in 1x PBS, 0.1% Tween-20 for 10 min, and washed twice in TN buffer (100 mM Tris-HCl pH 7.5, 150 mM NaCl) for 5 min at room temperature, followed by blocking in TNB buffer [100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.05% blocking reagent (Perkin Elmer)], and incubation with rabbit anti-DNP (Invitrogen) primary antibody diluted 1:600 in TNB buffer overnight at 4°C. .. Signal development proceeded with the tyramide signal amplification kit (Perkin Elmer), following the manufacturer's instructions.

Staining:

Article Title: Long-distance cell migration during larval development in the appendicularian, Oikopleura dioica.
Article Snippet: .. After washing 3 times (10 min per wash at RT) in Solution A (0.5 M NaCl/10 mM Tris HCl (pH 8.0)/50 mM EDTA/0.1% Tween), samples were treated with 100 mg/ml RNaseA in solution A at 37 1C for 2 h. The samples were then washed once in Solution A for 5 min, and 3 times in TN buffer (50 mM Tris–HCl (pH 7.4)/0.15 mM NaCl) for 10 min each time, and finally stained with 5 mM S7020 SYTOX Green (Invitrogen, Tokyo, Japan) in TN buffer at 4 1C overnight. .. After washing with TN buffer, the specimens were mounted in VECTASHIELD (Funakoshi, Tokyo, Japan).

Purification:

Article Title: Genome organization in double-stranded DNA viruses observed by cryoET
Article Snippet: Briefly, HSV-1 KOS strain (ATCC VR-1493) was cultured in Vero cells (ATCC CCL-81) using Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS). .. Virion particles were harvested from the culture media, concentrated by ultracentrifugation pelleting, purified with sucrose density gradient, and resuspended in TN buffer (20mM Tris-HCl pH7.4, 150mM NaCl) for cryoEM sample preparation. .. Bacteriophage T4 (ATCC 11303-B4), T7 (BAA-1025-B2) and their recommended host strains of Escherichia coli (ATCC 11303 for T4, and ATCC BAA-1025 for T7) were all purchased from ATCC and propagated following the standard protocol described on the ATCC website.

Sample Prep:

Northern Blot:

Article Title: The Allosteric HIV-1 Integrase Inhibitor BI-D Affects Virion Maturation but Does Not Influence Packaging of a Functional RNA Genome
Article Snippet: .. RNA for northern blotting was resuspended in TN buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl) and treated with DNase (Ambion) for 60 min at 37°C. ..

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1x tn buffer (Thermo Fisher)

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